Eubacterium limosum is a Gram positive, non-branching and non-spore forming rod that grows in anaerobic environment. Eubacterium spp are commonly seen as a normal flora in the gastrointestinal tract of humans but can cause bacteraemia in rare occasion.
This organism was isolated from the blood culture specimen of a hospital in-patient with clinical details of diarrhoea. The BD Bactec FX Blood culture analyser flagged the anaerobic bottle (pink top) positive after 56 hours incubation and upon Gram stain shows Gram positive non-branching rods. The specimen was then plated out on Blood agar, chocolate agar, UriSelect chromogenic agar and FAA AN anaerobic plates. All culture plates were purchased from E & O Labs (http://www.eolabs.com/). After 24 hours incubation at appropriate temperatures, there was growth on the FAA AN anaerobic plate only. The isolate shows a smooth, rounded, lustrous colony as seen on the culture plate above. This isolate was then identified using Biomerieux Vitek MS as Eubacterium limosum.